Proceedings of the National Academy of Sciences - PNAS, 2012-08, Vol.109 (32), p.E2173-E2182
2012
Details
- Autor(en) / Beteiligte
- Titel
- Global transcriptome response to ionic liquid by a tropical rain forest soil bacterium, Enterobacter lignolyticus
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 2012-08, Vol.109 (32), p.E2173-E2182
- Ort / Verlag
- United States: National Academy of Sciences
- Erscheinungsjahr
- 2012
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- To process plant-based renewable biofuels, pretreatment of plant feedstock with ionic liquids has significant advantages over current methods for deconstruction of lignocellulosic feedstocks. However, ionic liquids are often toxic to the microorganisms used subsequently for biomass saccharification and fermentation. We previously isolated Enterobacter lignolyticus strain SCF1, a lignocellulolytic bacterium from tropical rain forest soil, and report here that it can grow in the presence of 0.5 M 1-ethyl-3-methylimidazolium chloride, a commonly used ionic liquid. We investigated molecular mechanisms of SCF1 ionic liquid tolerance using a combination of phenotypic growth assays, phospholipid fatty acid analysis, and RNA sequencing technologies. Potential modes of resistance to 1-ethyl-3-methylimidazolium chloride include an increase in cyclopropane fatty acids in the cell membrane, scavenging of compatible solutes, up-regulation of osmoprotectant transporters and drug efflux pumps, and down-regulation of membrane porins. These findings represent an important first step in understanding mechanisms of ionic liquid resistance in bacteria and provide a basis for engineering microbial tolerance.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.1112750109Titel-ID: cdi_pubmed_primary_22586090
- Format
- –
- Schlagworte
- Bacteria, Base Sequence, Bioengineering - methods, Biofuels, Biological Sciences, biomass, cell membranes, Cells, Drug Resistance - physiology, engineering, Enterobacter, Enterobacter - drug effects, Enterobacter - growth & development, Enterobacter - metabolism, Fatty acids, feedstocks, Fermentation, Forest soils, Gene Expression Regulation, Bacterial - drug effects, Imidazoles, ionic liquids, Ionic Liquids - toxicity, lignocellulose, Membranes, Molecular Sequence Data, osmotolerance, phospholipid fatty acids, Phospholipids - metabolism, PNAS Plus, porins, Rainforests, RNA, saccharification, Sequence Analysis, RNA, soil bacteria, Soil Microbiology, solutes, toxicity, transcriptome, Transcriptome - drug effects, Transcriptome - genetics, transporters, Trees, Tropical Climate, tropical rain forests