Details

Autor(en) / Beteiligte

• Bertling, Anne
• Niemann, Silke
• Hussain, Muzaffar
• Holbrook, Lisa
• Stanley, Ronald G
• Brodde, Martin F
• Pohl, Silke
• Schifferdecker, Tina
• Roth, Joachim
• Jurk, Kerstin
• Müller, Anja
• Lahav, Judith
• Peters, Georg
• Heilmann, Christine
• Gibbins, Jonathan M
• Kehrel, Beate E

Titel

Staphylococcal extracellular adherence protein induces platelet activation by stimulation of thiol isomerases

Ist Teil von

• Arteriosclerosis, thrombosis, and vascular biology, 2012-08, Vol.32 (8), p.1979-1990

Ort / Verlag

United States

Erscheinungsjahr

2012

Quelle

MEDLINE

Beschreibungen/Notizen

• Staphylococcus aureus can induce platelet aggregation. The rapidity and degree of this correlates with the severity of disseminated intravascular coagulation, and depends on platelet peptidoglycans. Surface-located thiol isomerases play an important role in platelet activation. The staphylococcal extracellular adherence protein (Eap) functions as an adhesin for host plasma proteins. Therefore we tested the effect of Eap on platelets. We found a strong stimulation of the platelet-surface thiol isomerases protein disulfide isomerase and endoplasmic reticulum stress proteins 57 and 72 by Eap. Eap induced thiol isomerase-dependent glycoprotein IIb/IIIa activation, granule secretion, and platelet aggregation. Treatment of platelets with thiol blockers, bacitracin, and anti-protein disulfide isomerase antibody inhibited Eap-induced platelet activation. The effect of Eap on platelets and protein disulfide isomerase activity was completely blocked by glycosaminoglycans. Inhibition by the hydrophobic probe bis(1-anilinonaphthalene 8-sulfonate) suggested the involvement of hydrophobic sites in protein disulfide isomerase and platelet activation by Eap. In the present study, we found an additional and yet unknown mechanism of platelet activation by a bacterial adhesin, involving stimulation of thiol isomerases. The thiol isomerase stimulatory and prothrombotic features of a microbial secreted protein are probably not restricted to S aureus and Eap. Because many microorganisms are coated with amyloidogenic proteins, it is likely that the observed mechanism is a more general one.