Details

Autor(en) / Beteiligte

• Ji, Ying-Chang
• Li, Yu
• Lu, Feng
• Hu, Zhi-Qi
• Wang, Sen
• Lin, Chang-Min
• Gao, Jian-Hua

Titel

Preliminary study on inhibition of the hair follicle development by siRNA targeting Wnt10b in the cultured rat embryonic skin

Ist Teil von

• Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery, 2012-01, Vol.28 (1), p.39

Ort / Verlag

China

Erscheinungsjahr

2012

Quelle

MEDLINE

Beschreibungen/Notizen

• To investigate whether the suppression of Wnt10b by siRNA could prevent the development of hair follicle in the cultured rat embryonic skin. siRNA-Wnt10b was synthesized by chemosynthesis method. The dorsal skin of SD rat at embryos were cultured in DMEM in the presence of different percentage of interfering RNA targeting Wnt10b. Wnt10b/beta-catenin expression was analyzed by real-time PCR everyday and by Western blot on the third day. The cultured embryonic skin underwent paraffin embedding, section, HE staining on the third day,in which the number of de novo hair follicle was calculated and statistically analyzed. Wnt10b gene in the cultured embryonic skin could be knocked down with the siRNA-based method. Beta-catenin mRNA was not greatly influenced by the downregulation of Wnt10b mRNA. The number of de novo hair follicle placode in cultured embryonic skin decreased, along with the downregulation of Wnt10b and beta-catenin proteins expression. The downregulation of Wnt10b mRNA and protein by siRNA reduces