Zhonghua gan zang bing za zhi, 2011-06, Vol.19 (6), p.440
2011
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- Autor(en) / Beteiligte
- Titel
- The influence of HBV and its antigens on the expressions of JAK-STAT signal transduction pathway molecules and antiviral proteins of IFN alpha
- Ist Teil von
- Zhonghua gan zang bing za zhi, 2011-06, Vol.19 (6), p.440
- Ort / Verlag
- China
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To investigate the possible influence of HBV and its antigens on the expressions of JAK-STAT signal transduction pathway molecules and the antiviral proteins of IFN alpha. The HepG2 cells were transfected with pSM2, pHBS2-S and pHBc-EGFP plasmids which express HBV whole particles or S-antigen, Pre-S antigen and core antigens. The infectious supernatant from HepG2.2.15 cells and the pured HBV proteins which contained the S, Pre-S antigens were used to treat the HepG2 cells. Northern blot and RT-PCR were applied to analyse the expressions of the antiviral proteins MxA, 2' -5' OAS, 9-27 and the JAK-STAT signal transduction pathway molecules STAT1 in HepG2 cells responded to the IFN alpha treatment. The HepG2 cells transfected with pSM2, pHBS2-S and pHBc-EGFP plasmids could express whole HBV particles and HBsAg, Pre-S antigen and HBcAg. The quantitation of expressed HBV particles and antigens increased significantly during the course of transfection. Northern blot hybridization analysis indicated that the HepG2 c
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 1007-3418Titel-ID: cdi_pubmed_primary_22053375
- Format
- –
- Schlagworte
- 2',5'-Oligoadenylate Synthetase - metabolism, GTP-Binding Proteins - metabolism, Hep G2 Cells, Hepatitis B Antigens - immunology, Hepatitis B virus - immunology, Humans, Interferon-alpha - metabolism, Myxovirus Resistance Proteins, RNA-Binding Proteins - metabolism, Signal Transduction, STAT1 Transcription Factor - metabolism, Transfection