Details

Autor(en) / Beteiligte

• Cui, Jing-jing
• Zhu, Xin-long
• Ji, Chang-fu
• Jing, Xiang-hong
• Bai, Wan-zhu

Titel

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Ist Teil von

• Zhēncì yánjiū, 2011-08, Vol.36 (4), p.262

Ort / Verlag

China

Erscheinungsjahr

2011

Quelle

MEDLINE

Beschreibungen/Notizen

• To study the specific correlation between "Jinggu" (BL 64) and "Dazhong" (KI 4) in the nervous system by using a double-labeling of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594 (CTB-Alexa 488, 594) in rats, so as to investigate its neuroanatomical basis for clinical joint-application of Yuan-Source and Luo acupoints. Three male SD rats were used in the present study. Under anesthesia (10% urethane), 0.1% CTB-Alexa 488 (5 microL) and CTB-Alexa 594 (5 microL) were respectively injected into the border area between the red and white flesh, distal to the tuberosity of the fifth metatarsal bone, and the depression anterior to the medial attachment of the calcaneal tendon, the corresponding sites of the acupoints Jinggu (BL 64) and Dazhong (KI 4) in the human body. After 3 surviving days, the rat's brain, spinal cord and dorsal root ganglia (DRGs) of L3-L6 were dissected following perfusion with 4% paraformaldehyde, cut into sections and observed under fluorescent microscope equipped with a digit