Nature methods, 2011-10, Vol.8 (10), p.837-839
2011
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Details
- Autor(en) / Beteiligte
- Titel
- Light-based feedback for controlling intracellular signaling dynamics
- Ist Teil von
- Nature methods, 2011-10, Vol.8 (10), p.837-839
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The ability to apply precise inputs to signaling species in live cells would be transformative for interrogating and understanding complex cell-signaling systems. Here we report an 'optogenetic' method for applying custom signaling inputs using feedback control of a light-gated protein-protein interaction. We applied this strategy to perturb protein localization and phosphoinositide 3-kinase activity, generating time-varying signals and clamping signals to buffer against cell-to-cell variability or changes in pathway activity.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1548-7091eISSN: 1548-7105DOI: 10.1038/nmeth.1700Titel-ID: cdi_pubmed_primary_21909100
- Format
- –
- Schlagworte
- Animals, Cell Membrane - drug effects, Cell Membrane - metabolism, Computational Biology, Feedback, Physiological - radiation effects, Humans, Light, Models, Biological, Phosphatidylinositol 3-Kinases - chemistry, Phosphatidylinositol 3-Kinases - metabolism, Phosphoinositide-3 Kinase Inhibitors, Protein Binding, Signal Transduction - radiation effects