Journal of medicinal food, 2011-10, Vol.14 (10), p.1181
2011
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- Autor(en) / Beteiligte
- Titel
- Alcohol-fermented soybean increases the expression of receptor-interacting protein 2 and IκB kinase β in mouse peritoneal macrophages
- Ist Teil von
- Journal of medicinal food, 2011-10, Vol.14 (10), p.1181
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Soybean is a useful component of traditional Korean medicine with well-documented health-promoting effects. We investigated the effects of alcohol-fermented soybean (AFS) on immune function. When AFS treatment was used in combination with recombinant interferon-γ (rIFN-γ), there was a marked cooperative induction of nitric oxide (NO) and tumor necrosis factor (TNF)-α production in mouse peritoneal macrophages. AFS increased the expression of inducible NO synthase mRNA and protein in rIFN-γ-primed macrophages. Treating macrophages with pyrrolidine dithiocarbamate, an inhibitor of nuclear factor-κB (NF-κB), decreased the synergistic effects of AFS. In addition, AFS in combination with rIFN-γ increased the phosphorylation of p38 and c-Jun N-terminal kinase (JNK) but not extracellular signal-regulated kinase. However, AFS had no effect on phosphorylation of mitogen-activated protein kinases by itself. The p38 inhibitor SB203580 or the JNK inhibitor SP600125 inhibited the AFS-induced NO and TNF-α production. When AFS was used in combination with rIFN-γ, there was a co-operative activation of NF-κB and receptor-interacting protein 2 (Rip2)/IκB kinase (IKK)-β. Our results indicate that AFS increases the production of NO and TNF-α through the activation of Rip2/IKK-β in rIFN-γ-primed macrophages.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 1557-7600DOI: 10.1089/jmf.2010.1522Titel-ID: cdi_pubmed_primary_21895414
- Format
- –
- Schlagworte
- Animals, Blotting, Western, Cyclooxygenase 2 - genetics, Cyclooxygenase 2 - metabolism, Extracellular Signal-Regulated MAP Kinases - genetics, Extracellular Signal-Regulated MAP Kinases - metabolism, Fermentation, Glycine max - metabolism, I-kappa B Kinase - genetics, I-kappa B Kinase - metabolism, Interferon-gamma - metabolism, JNK Mitogen-Activated Protein Kinases - metabolism, Macrophages, Peritoneal - drug effects, Male, Mice, Mice, Inbred C57BL, NF-kappa B - genetics, NF-kappa B - metabolism, Nitric Oxide - biosynthesis, Nitric Oxide Synthase Type II - genetics, Nitric Oxide Synthase Type II - metabolism, Phosphorylation, Pyrrolidines - metabolism, Receptor-Interacting Protein Serine-Threonine Kinase 2, Receptor-Interacting Protein Serine-Threonine Kinases - genetics, Receptor-Interacting Protein Serine-Threonine Kinases - metabolism, Reverse Transcriptase Polymerase Chain Reaction, Thiocarbamates - metabolism, Tumor Necrosis Factor-alpha - biosynthesis, Tumor Necrosis Factor-alpha - genetics