Details

Autor(en) / Beteiligte

• Li, Yong-guang
• Li, Wen-hui
• Gai, Wen-yan
• Yao, Ju-xia
• Qu, Zi-gang
• Jia, Wan-zhong
• Radu, Blaga
• Fu, Bao-quan

Titel

Cloning and sequence analysis of thioredoxin peroxidase gene from Taenia multiceps

Ist Teil von

• Zhongguo jishengchongxue yu jishengchongping zazhi, 2011-02, Vol.29 (1), p.67

Ort / Verlag

China

Erscheinungsjahr

2011

Quelle

MEDLINE

Beschreibungen/Notizen

• Protoscoleces of Taenia multiceps were collected from the naturally infected sheep and total RNA was extracted. Specific primers were designed according to TaHe2-D11 mRNA sequence and T. multiceps thioredoxin peroxidase gene (TmTPx) was amplified by RT-PCR. PCR products were ligated into pMD18-T vector and transformed to E. coli DH5alpha. The recombinant plasmids were identified by restriction digestion and sequencing. A 614 bp cDNA was amplified. The TmTPx open reading frame (591 bp) encoded a 196-amino acid protein with Mr 21,690, pI 7.61. Bioinformatics analysis indicated that TmTPx had a typical 2-Cys Prx conserved domain. Phylogenetic tree revealed that T. multiceps had the closest relationship to T. asiatica, followed by T. solium and T. crassiceps, E. granulosus and E. multilocularis.