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Morphological study on commonly-used acupoints for thyroid surgery under acupuncture anesthesia
Ist Teil von
Zhēncì yánjiū, 2011-06, Vol.36 (3), p.199
Ort / Verlag
China
Erscheinungsjahr
2011
Quelle
MEDLINE
Beschreibungen/Notizen
To study the morphologic structure, afferent projections of "Neiguan" (PC6), "Hegu" (LI 4) and "Futu" (LI 18) acupoint regions so as to understand their underlying mechanisms in acupuncture anesthesia for thyroid surgery.
A total of 66 Wistar rats were used in the present study. (1) Horseradish peroxidase (HRP, 10%, 10 microL) was injected into PC 6, LI 4 and LI 18 acupoints and the thyroid area on the right side, respectively (n = 6). (2) Propidium iodide (PI, 10% 10 microL) or Bisbenzimide (Bb, 5%, 10 microL) was injected into above-mentioned 3 acupoints and the thyroid region on the right side, respectively (n = 6). The numbers and percentages of the labeled neurons in the dorsal root ganglia (DRGs, C 1 - C 8 and T 1) were observed under microscope. (3) Cutaneous connexin (Cx) 43 immunoactivity of LI 18, PC 6, LI 4 and "Zusanli" (ST 36) regions and their individual non-acupoint regions (about 0.5 cm lateral to LI 18, PC 6, LI 4, and ST 36, n = 12) was determined by immunohistochemistry.
1) HRP-labeled neur