Chung-hua nei kʿo tsa chih, 2011-03, Vol.50 (3), p.235
2011
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- Autor(en) / Beteiligte
- Titel
- The effects of soluble epoxide hydrolase inhibitors on cholesterol efflux in adipocytes
- Ist Teil von
- Chung-hua nei kʿo tsa chih, 2011-03, Vol.50 (3), p.235
- Ort / Verlag
- China
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To observe the effects of soluble epoxide hydrolase inhibitors tAUCB on cholesterol efflux in adipocytes. 3T3-L1 preadipocytes were induced to differentiation and maturation. Cells were stimulated with 100 µg/L LPS after starved for 24 hours, then tAUCB in various concentrations (1, 10, 50, 100 µmol/L)were added for 24 h, or incubated with the peroxisome proliferator activated receptor gamma (PPARγ) antagonist GW9662 (5 µmol/L). 0 µmol/L tAUCB treated group was taken as empty control. After then, the mRNA expression of PPARγ and adenosine triphosphate binding cassette transporter A1(ABCA1) in cells were determined via realtime-PCR, the amounts of protein expression of PPARγ and ABCA1 in cells were detected by Western blot, the efflux rates of (3)H-cholesterol in cells were detected by means of liquid scintillation counter. tAUCB could dose-dependently increase the apolipoprotein A1 (apoA1)-mediated cholesterol efflux in adipocytes. After stimulated by 1, 10, 50, 100 µmol/L tAUCB, cholesterol efflux rates were
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 0578-1426Titel-ID: cdi_pubmed_primary_21600089
- Format
- –
- Schlagworte
- 3T3-L1 Cells, Adipocytes - cytology, Adipocytes - drug effects, Adipocytes - metabolism, Animals, Apolipoprotein A-I - genetics, Apolipoprotein A-I - metabolism, Benzoates - pharmacology, Cholesterol - metabolism, Enzyme Inhibitors - pharmacology, Epoxide Hydrolases - antagonists & inhibitors, Mice, PPAR gamma - genetics, PPAR gamma - metabolism, RNA, Messenger - genetics, Urea - analogs & derivatives, Urea - pharmacology