Details

Autor(en) / Beteiligte

• Park, Kwang-Hyun
• Kim, Byung-Ju
• Kang, John
• Nam, Tae-Sik
• Lim, Jung Min
• Kim, Hyoung Tae
• Park, Jong Kwan
• Kim, Young Gon
• Chae, Soo-Wan
• Kim, Uh-Hyun

Titel

Ca2+ signaling tools acquired from prostasomes are required for progesterone-induced sperm motility

Ist Teil von

• Science signaling, 2011-05, Vol.4 (173), p.ra31

Ort / Verlag

United States

Erscheinungsjahr

2011

Quelle

MEDLINE

Beschreibungen/Notizen

• Progesterone-induced calcium ion (Ca2+) signals in the neck region of sperm play a pivotal role in promoting sperm motility. Here, we show that a long-lasting Ca2+ signal required for sperm motility in response to progesterone depends on their pH-dependent fusion with prostasomes, which are small vesicles secreted by the prostate. We found that prostasome fusion led to the transfer of progesterone receptors, cyclic adenosine diphosphoribose (cADPR)-synthesizing enzymes, ryanodine receptors (RyRs), and other Ca2+ signaling tools from prostasomes to the sperm neck. Progesterone-induced sperm motility relied on cADPR-mediated Ca2+ mobilization through RyR located on acidic Ca2+ stores, followed by Ca2+ entry through store-operated channels. Treatment of prostasome-fused sperm with a cADPR antagonist or fusion with prostasomes in which type 2 RyR was depleted resulted in low fertilization rates, reduced sperm motility, or both. Thus, we conclude that sperm motility depends on the acquisition of Ca2+ signaling tools from prostasomes.