Details

Autor(en) / Beteiligte

• Popot, J.-L
• Althoff, T
• Bagnard, D
• Banères, J.-L
• Bazzacco, P
• Billon-Denis, E
• Catoire, L.J
• Champeil, P
• Charvolin, D
• Cocco, M.J
• Crémel, G
• Dahmane, T
• de la Maza, L.M
• Ebel, C
• Gabel, F
• Giusti, F
• Gohon, Y
• Goormaghtigh, E
• Guittet, E
• Kleinschmidt, J.H
• Kühlbrandt, W
• Le Bon, C
• Martinez, K.L
• Picard, M
• Pucci, B
• Sachs, J.N
• Tribet, C
• van Heijenoort, C
• Wien, F
• Zito, F
• Zoonens, M

Titel

Amphipols From A to Z

Ist Teil von

• Annual review of biophysics, 2011-06, Vol.40 (1), p.379-408

Ort / Verlag

United States: Annual Reviews

Erscheinungsjahr

2011

Quelle

MEDLINE

Beschreibungen/Notizen

• Amphipols (APols) are short amphipathic polymers that can substitute for detergents to keep integral membrane proteins (MPs) water soluble. In this review, we discuss their structure and solution behavior; the way they associate with MPs; and the structure, dynamics, and solution properties of the resulting complexes. All MPs tested to date form water-soluble complexes with APols, and their biochemical stability is in general greatly improved compared with MPs in detergent solutions. The functionality and ligand-binding properties of APol-trapped MPs are reviewed, and the mechanisms by which APols stabilize MPs are discussed. Applications of APols include MP folding and cell-free synthesis, structural studies by NMR, electron microscopy and X-ray diffraction, APol-mediated immobilization of MPs onto solid supports, proteomics, delivery of MPs to preexisting membranes, and vaccine formulation.