Details

Autor(en) / Beteiligte

• Gu, Hai-Yan
• Zhao, De-Yu
• Wang, Quan

Titel

Effect of maternal vitamin D deficiency on lung morphogenesis and platelet-derived growth factor-A expression in rat offspring

Ist Teil von

• Zhongguo dang dai er ke za zhi, 2011-04, Vol.13 (4), p.326

Ort / Verlag

China

Erscheinungsjahr

2011

Quelle

MEDLINE

Beschreibungen/Notizen

• To study the effect of maternal vitamin D deficiency on lung morphogenesis and platelet-derived growth factor-A (PDGF-A) expression in rat offspring. Sprague-Dawley (SD) female rats were randomly divided into two groups: normal control and vitamin D deficiency, with 6 rats in each group. The vitamin D deficiecy group was kept away from light and fed with the forage without vitamin D. After 2 weeks, the rats were mated with normal SD male rats. The morphological changes of fetal rat lungs on day 20 of gestation and 1-day-old neonatal rat lungs were observed by light microscope and electronic microscope. The levels of PDGF-A mRNA and protein in fetal and neonatal rat lungs were measured by reverse transcriptase-polymerase chain reaction (RT-PCR) technique and Western blot method respectively. Under the light microscope, smaller alveolar space, smaller diameter of the respiratory membrane and thicker alveolus mesenchyma were observed in lungs of fetal and neonatal rats from the vitamin D deficiency group compare