Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban, 2011-03, Vol.42 (2), p.190
2011
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- Autor(en) / Beteiligte
- Titel
- The role of DNA double strains damage repairing mechanisms in high glucose-induced endothelial senescence
- Ist Teil von
- Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban, 2011-03, Vol.42 (2), p.190
- Ort / Verlag
- China
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To investigate the roles of DNA double stains damage repairing mechanisms in high glucose-induced cellular senescence. Human umbilical vein endothelial cells (HUVECs) were incubated with different concentrations of glucose (5.5 mmol/L, 11 mmol/L, 22 mmol/L and 33 mmol/L) for 72 hrs before the assay of senescence-associated beta-galactosidase staining. The superoxides were detected by flow cytometry. The levels of NO were detected by enzyme assay. Gamma-H2AX and phosphorylated P53 protein were measured by Western blot. Changes after co-incubation with KU55993 (an inhibitor of ATM) were examined with methods mentioned above. Compared with control group, percentage of positive cells of senescence-associated beta-galactosidase staining increased significantly in high glucose groups. The corresponding levels of reactive oxygen increased and NO decreased in a concentration-dependent manner. Intra-cellular levels of gamma-H2AX and phosphorylated P53 protein were significantly increased in high glucose groups. Statis
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 1672-173XTitel-ID: cdi_pubmed_primary_21500551
- Format
- –
- Schlagworte
- beta-Galactosidase - metabolism, Cells, Cultured, Cellular Senescence - drug effects, DNA Breaks, Double-Stranded - drug effects, DNA Damage - drug effects, Glucose - pharmacology, Histones - metabolism, Human Umbilical Vein Endothelial Cells - metabolism, Human Umbilical Vein Endothelial Cells - pathology, Humans, Morpholines, Nitric Oxide - analysis, Pyrones, Tumor Suppressor Protein p53 - metabolism