Details

Autor(en) / Beteiligte

• Grosse-Wilde, Ewald
• Kuebler, Linda S
• Bucks, Sascha
• Vogel, Heiko
• Wicher, Dieter
• Hansson, Bill S

Titel

Antennal transcriptome of Manduca sexta

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 2011-05, Vol.108 (18), p.7449-7454

Ort / Verlag

United States: National Academy of Sciences

Erscheinungsjahr

2011

Quelle

MEDLINE

Beschreibungen/Notizen

• In recent years, considerable progress has been made in understanding the molecular mechanisms underlying olfaction in insects. Because of the diverse nature of the gene families involved, this process has largely relied on genomic data. As a consequence, studies have focused on a small subset of species with extensive genomic information. For Lepidoptera, a large order historically crucial to olfactory research, this circumstance has mostly limited advances to the domesticated species Bombyx mori, with some progress in the noctuid Heliothis virescens based on a nonpublic partial genome database. Because of the limited behavioral repertoire and nonexistent ecological importance of Bombyx, molecular data on the tobacco hornworm Manduca sexta are of utmost importance, especially with regards to its position as a classical olfactory model and its complex natural behavior. Here we present the use of transcriptomic and microarray data to identify members of the main olfactory gene families of MANDUCA: To assess the quality of our data, we correlate information on expressed receptor genes with detailed morphological data on the antennal lobe. Finally, we compare the expression of the near-complete transcript sets in male and female antennae.