Neoplasma, 2011, Vol.58 (3), p.181-188
2011
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Details
- Autor(en) / Beteiligte
- Titel
- Expression of soluble TGF-β receptor II by recombinant Vaccinia virus enhances E7 specific immunotherapy of HPV16 tumors
- Ist Teil von
- Neoplasma, 2011, Vol.58 (3), p.181-188
- Ort / Verlag
- Slovakia
- Erscheinungsjahr
- 2011
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Therapeutic immunization with double recombinants of vaccinia virus (VACV) co-expressing sTβRII increased rejection of established TC-1 tumors in C57BL/6 mice in comparison with single recombinant expressing SigE7LAMP. Recombinant VACV derived from vaccination strain Praha expressed either the sTβRII (ectodomain) or chimeric protein fused to immunoglobulin Fc fragment (sTβRII-Fc-Jun) under control of two different promotors together with the immunogenic tumor associated antigen HPV16 E7 oncoprotein in a form of SigE7LAMP fusion molecule. The ability of soluble receptors to bind TGF-β in vitro was proved. Immunization of mice with double recombinant viruses and virus expressing SigE7LAMP only led to eliciting similar response of E7 specific CD8+ T cells as detected by IFN-γ ELISPOT.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0028-2685DOI: 10.4149/neo_2011_03_181Titel-ID: cdi_pubmed_primary_21391733
- Format
- –
- Schlagworte
- Animals, Female, Human papillomavirus 16 - immunology, Immunization, Mice, Mice, Inbred C57BL, Neoplasms, Experimental - therapy, Papillomavirus E7 Proteins - immunology, Papillomavirus Vaccines - immunology, Protein-Serine-Threonine Kinases - genetics, Receptors, Transforming Growth Factor beta - genetics, T-Lymphocytes - immunology, Vaccines, Synthetic - immunology, Vaccinia virus - genetics