Details

Autor(en) / Beteiligte

• Shuvaeva, G P
• Sysoeva, M G

Titel

Xylanase of the micromycete Rhizopus var. microsporus 595: preparation, structural and functional characteristics, and application

Ist Teil von

• Prikladnaja biohimija i mikrobiologija, 2010-11, Vol.46 (6), p.693

Ort / Verlag

Russia (Federation)

Erscheinungsjahr

2010

Quelle

MEDLINE

Beschreibungen/Notizen

• Procedures for the production of endo-1,4-beta-xylanase have been developed. An active producer-Rhizopus var. microsporus BKMF-595-has been chosen, and the conditions of surface and submerged cultivation, as well as the composition of the culture medium for this strain, have been optimized to ensure maximum yield of the target enzyme. Activity of xylomicrosporin Px equaled 123 U/g, while the activity of xylomicrosporin Gx equaled 25 U/cm3. Homogeneous enzyme preparations, purified 59.44-fold and 72.6-fold, have been obtained. The dependence of endo-1,4-beta-xylanase catalytic activity on temperature and pH of the reaction medium has been studied. The enzyme has been shown to be most stable in the pH range 5.0-6.0 and to be thermostable. Amino acid composition and subunit structure of the enzyme were determined; the molecular masses of the subunits equaled 50 and 56 kDa. Carboxyl groups of glutamic and aspartic acid residues of the active center were experimentally shown to play an important role in catalysis. The potential of this enzyme for beer production has been demonstrated.