Details

Autor(en) / Beteiligte

• Chu, Youjun
• Yao, Phil Y
• Wang, Wenwen
• Wang, Dongmei
• Wang, Zhikai
• Zhang, Liangyu
• Huang, Yuejia
• Ke, Yuwen
• Ding, Xia
• Yao, Xuebiao

Titel

Aurora B kinase activation requires survivin priming phosphorylation by PLK1

Ist Teil von

• Journal of molecular cell biology, 2011-08, Vol.3 (4), p.260-267

Ort / Verlag

United States

Erscheinungsjahr

2011

Quelle

MEDLINE

Beschreibungen/Notizen

• During cell division, chromosome segregation is orchestrated by the interaction of spindle microtubules with the centromere. Accurate attachment of spindle microtubules to kinetochore requires the chromosomal passenger of Aurora B kinase complex with borealin, INCENP and survivin (SUR). The current working model argues that SUR is responsible for docking Aurora B to the centromere whereas its precise role in Aurora B activation has been unclear. Here, we show that Aurora B kinase activation requires SUR priming phosphorylation at Ser20 which is catalyzed by polo-like kinase 1 (PLK1). Inhibition of PLK1 kinase activity or expression of non-phosphorylatable SUR mutant prevents Aurora B activation and correct spindle microtubule attachment. The PLK1-mediated regulation of Aurora B kinase activity was examined in real-time mitosis using fluorescence resonance energy transfer-based reporter and quantitative analysis of native Aurora B substrate phosphorylation. We reason that the PLK1-mediated priming phosphorylation is critical for orchestrating Aurora B activity in centromere which is essential for accurate chromosome segregation and faithful completion of cytokinesis.