Nature methods, 2010-12, Vol.7 (12), p.989
2010
Details
- Autor(en) / Beteiligte
- Titel
- A defined glycosaminoglycan-binding substratum for human pluripotent stem cells
- Ist Teil von
- Nature methods, 2010-12, Vol.7 (12), p.989
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2010
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To exploit the full potential of human pluripotent stem cells for regenerative medicine, developmental biology and drug discovery, defined culture conditions are needed. Media of known composition that maintain human embryonic stem (hES) cells have been developed, but finding chemically defined, robust substrata has proven difficult. We used an array of self-assembled monolayers to identify peptide surfaces that sustain pluripotent stem cell self-renewal. The effective substrates displayed heparin-binding peptides, which can interact with cell-surface glycosaminoglycans and could be used with a defined medium to culture hES cells for more than 3 months. The resulting cells maintained a normal karyotype and had high levels of pluripotency markers. The peptides supported growth of eight pluripotent cell lines on a variety of scaffolds. Our results indicate that synthetic substrates that recognize cell-surface glycans can facilitate the long-term culture of pluripotent stem cells.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 1548-7105DOI: 10.1038/nmeth.1532Titel-ID: cdi_pubmed_primary_21076418
- Format
- –
- Schlagworte
- Amino Acid Sequence, Binding Sites, Cell Adhesion, Cell Differentiation, Glycosaminoglycans - metabolism, Heparin - metabolism, Humans, Peptide Fragments - chemistry, Peptide Fragments - metabolism, Peptides - metabolism, Pluripotent Stem Cells - cytology, Pluripotent Stem Cells - metabolism, Pluripotent Stem Cells - physiology, Protein Binding, Receptors, Cell Surface - metabolism, Stem Cells - cytology, Stem Cells - physiology, Substrate Specificity