Details

Autor(en) / Beteiligte

• Solov'eva, T F
• Bakholdina, S I
• Ermak, I M
• Khomenko, V A
• Fedoreeva, L I
• Novikova, O D
• Frolova, G M
• Likhatskaia, G N
• Ovodov, Iu S

Titel

Features of a protein component of the endotoxin from Yersinia pseudotuberculosis

Ist Teil von

• Bioorganicheskaia khimiia, 1990-10, Vol.16 (10), p.1301

Ort / Verlag

Russia (Federation)

Erscheinungsjahr

1990

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The protein moiety of endotoxin from Yersinia pseudotuberculosis was found to consist of two polypeptides with apparent molecular masses 40 and 14.5 kDa (4:1 w/w). The major protein (40 kDa) was isolated from the endotoxin pretreated with sodium deoxy cholate by gel chromatography on the Sephadex G-200 column. Comparative study of this protein and oligomeric form of porin from the outer membrane of Y. pseudotuberculosis using SDS--PAGE, velocity sedimentation, lipid bilayer experiments, chemical and serological analyses revealed their identity. The deoxycholate treatment of the endotoxin does not affect complexes of the major protein and LPS.