Details

Autor(en) / Beteiligte

• Nishimura, Y
• Fukuoka, N
• Fukuyoshi, Y
• Matsuo, Y
• Yamaguchi, K
• Takatsuki, K

Titel

Evaluation of specificity of EIA kit (ED-007)

Ist Teil von

• Rinsho byori. The Japanese journal of clinical pathology, 1990-12, Vol.38 (12), p.1363

Ort / Verlag

Japan

Erscheinungsjahr

1990

Quelle

MEDLINE

Beschreibungen/Notizen

• EIA (Eitest: Eisai) and Gelatin Particle Agglutination: PA (SERODIA: Fujirebio) assays were performed for the detection of HTLV-I antibodies with 10,780 sera from patients since 1986. Eleven point five percent were reactive by both EIA and PA, while 1.1% was PA(+), EIA(-), these PA false positive occurred on low titer. Conversely 0.2% on EIA positive seems PA false negative because of Western blot (WB) positivity. Zero point nine percent was EIA(+), PA(-), of these 80.6% were not inhibited by EIA confirmatory test. The main cause of EIA false positive was due to reactivity of auto antibody with MT-2 cell lysate. We used new EIA (ED-007: Eisai) coated with HTLV-I antigen purified from supernatant of culture medium of MT-2 cell. The results completely matched with EIA confirmatory test and WB. Cut off index value of sera from SLE patients were down to 0.2 (mean +/- SD) from 0.7 +/- 0.4 of Eitest ATL. EIA (ED-007) are probably useful and more specific assays for the detection of HTLV-I antibodies, especially, the sera of patients with autoimmune diseases.