Details

Autor(en) / Beteiligte

• Horikawa, Kazuki
• Yamada, Yoshiyuki
• Matsuda, Tomoki
• Kobayashi, Kentarou
• Hashimoto, Mitsuhiro
• Matsu-ura, Toru
• Miyawaki, Atsushi
• Michikawa, Takayuki
• Mikoshiba, Katsuhiko
• Nagai, Takeharu

Titel

Spontaneous network activity visualized by ultrasensitive Ca(2+) indicators, yellow Cameleon-Nano

Ist Teil von

• Nature methods, 2010-09, Vol.7 (9), p.729

Ort / Verlag

United States

Erscheinungsjahr

2010

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• We report ultrasensitive Ca(2+) indicators, yellow cameleon-Nano (YC-Nano), developed by engineering the Ca(2+)-sensing domain of a genetically encoded Ca(2+) indicator, YC2.60 or YC3.60. Their high Ca(2+) affinities (K(d) = 15-140 nM) and large signal change (1,450%) enabled detection of subtle Ca(2+) transients associated with intercellular signaling dynamics and neuronal activity, even in 100,000-cell networks. These indicators will be useful for studying information processing in living multicellular networks.