Details

Autor(en) / Beteiligte

• Sheng, Wei
• Chang, Li
• Zhang, Aimin
• Xue, Jianping
• Liu, Yuling

Titel

Research on callus induction and plantlet regeneration of Prunella vulgaris

Ist Teil von

• Zhongguo zhongyao zazhi, 2010-04, Vol.35 (7), p.830

Ort / Verlag

China

Erscheinungsjahr

2010

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• To study the conditions of callus induction and plantlet regeneration of Prunella vulgaris. By using the orthogonal experiment design, different explants, sucrose, plant growth substances and their ratio for callus induction and differentiation were optimized. The inductivity of leaves was the highest, followed by stems, but callus of the leafstalks could not be induced. The main factor of affecting the formation of callus was 6-BA. The optimal medium for callus induction was MS + 6-BA 3.0 mg x L(-1) + NAA 0.1 mg x L(-1) + 2,4-D 0.5 mg x L(-1) + sucrose 3%. The optimal medium for callus differentiation was 1/2 MS + 6-BA 3.25 mg x L(-1) + NAA 1.25 mg x L(-1) + sucrose 2%. The optimal medium for rooting was MS + IBA 1.0 mg x L(-1) + sucrose 3%.