Xi bao yu fen zi mian yi xue za zhi, 2010-04, Vol.26 (4), p.333
2010
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Details
- Autor(en) / Beteiligte
- Titel
- Expression of human HMGB1 A box in E. coli and its inhibitory effects on monocytes
- Ist Teil von
- Xi bao yu fen zi mian yi xue za zhi, 2010-04, Vol.26 (4), p.333
- Ort / Verlag
- China
- Erscheinungsjahr
- 2010
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To clone human high mobility guoup box1 A box (HMGB1 A box) and express it in escherichia coli effectly, investigate the inhibit effection of the purpose protern to the activation of monocytes stimulated by immunocomplex. According to human HMGB1 gene order which was optimized by our laboratory the PCR primer was designed which containing restriction enzyme cutting site. The HMGB1 A box gene was cloned following the whole gene synthesis template of human HMGB1, then the PCR product was inserted into clone vector pMD19-T. The positive colone was identified by colony PCR, zymography analysis and DNA sequencing. Recombinant colne vector was digested by restriction enzymes Nde I and Xho I and separated by agarose gel electrophoresis, then the fragment was inserted into the corresponding sites of expression vector pQE-T7-2. The positive recombinant expression vector was identified by colony PCR and the recombinant strains was induced by IPTG, then the purpose protein was identified by SDS-PAGE and Western blot. Th
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 1007-8738Titel-ID: cdi_pubmed_primary_20368109
- Format
- –
- Schlagworte
- Cell Line, Escherichia coli - genetics, Gene Expression, Genetic Engineering - methods, Genetic Vectors - genetics, HMGB1 Protein - biosynthesis, HMGB1 Protein - genetics, HMGB1 Protein - isolation & purification, HMGB1 Protein - pharmacology, Humans, Monocytes - drug effects, Monocytes - immunology, Recombinant Proteins - biosynthesis, Recombinant Proteins - genetics, Recombinant Proteins - isolation & purification, Recombinant Proteins - pharmacology