Cold Spring Harbor protocols, 2009-02, Vol.2009 (2), p.pdb.prot5143
2009
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Details
- Autor(en) / Beteiligte
- Titel
- Transformation of the moss Physcomitrella patens using direct DNA uptake by protoplasts
- Ist Teil von
- Cold Spring Harbor protocols, 2009-02, Vol.2009 (2), p.pdb.prot5143
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2009
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- INTRODUCTION This protocol describes how to transform moss ( Physcomitrella patens ) protoplasts using polyethylene glycol (PEG)-mediated DNA uptake. The transformation rates for direct uptake by protoplasts of DNA with and without genomic sequence (a targeting construct) are typically 10 −5 and 10 −3 , respectively. (These are the frequencies of stable transformants among regenerants surviving the transformation procedure.)
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1940-3402eISSN: 1559-6095DOI: 10.1101/pdb.prot5143Titel-ID: cdi_pubmed_primary_20147073
- Format
- –
- Schlagworte
- Bryopsida - cytology, Bryopsida - genetics, DNA - genetics, DNA - pharmacokinetics, Plants, Genetically Modified, Polyethylene Glycols - pharmacology, Protoplasts - cytology, Protoplasts - metabolism, Surface-Active Agents - pharmacology, Transformation, Genetic - drug effects, Transformation, Genetic - genetics