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Establishment of Chinese hamster ovary cell line expressing recombinant GPIb-IX complex
Ist Teil von
Zhongguo shi yan xue ye xue za zhi, 2009-12, Vol.17 (6), p.1560
Ort / Verlag
China
Erscheinungsjahr
2009
Quelle
MEDLINE
Beschreibungen/Notizen
The aim of this study was to construct Chinese Hamster Ovary (CHO) cell models expressing recombinant wild-type GPIb-IX and mutant GPIb-IX complex, so as to provide the platform to study the related physiologic functions of GPIb-IX. The plasmids were extracted from E.coli expressing wild-type or deletion mutant GPIbalpha and were identified by digestion with EcoR I. Three plasmids containing GPIbalpha, GPIbbeta, and GPIX genes were co-transfected into CHO cells, and then the expression of GPIb-IX complex was detected by immune coprecipitation, Western blot and flow cytometry. The results showed that the expression of GPIb-IX complex could be detected in the lysate and on the surface of CHO cells at 48 hours after transfection. In conclusion, CHO cell models expressing recombinant wild-type or mutation GPIb-IX complex has been successfully constructed.