Details

Autor(en) / Beteiligte

• Liao, Yi
• Zhang, Wei-Lin
• Yuan, Yan-Hong
• Shi, Quan-Wei
• Li, Su-Ping
• Yan, Rong
• Wang, Zhi-Cheng
• Dai, Ke-Sheng

Titel

Establishment of Chinese hamster ovary cell line expressing recombinant GPIb-IX complex

Ist Teil von

• Zhongguo shi yan xue ye xue za zhi, 2009-12, Vol.17 (6), p.1560

Ort / Verlag

China

Erscheinungsjahr

2009

Quelle

MEDLINE

Beschreibungen/Notizen

• The aim of this study was to construct Chinese Hamster Ovary (CHO) cell models expressing recombinant wild-type GPIb-IX and mutant GPIb-IX complex, so as to provide the platform to study the related physiologic functions of GPIb-IX. The plasmids were extracted from E.coli expressing wild-type or deletion mutant GPIbalpha and were identified by digestion with EcoR I. Three plasmids containing GPIbalpha, GPIbbeta, and GPIX genes were co-transfected into CHO cells, and then the expression of GPIb-IX complex was detected by immune coprecipitation, Western blot and flow cytometry. The results showed that the expression of GPIb-IX complex could be detected in the lysate and on the surface of CHO cells at 48 hours after transfection. In conclusion, CHO cell models expressing recombinant wild-type or mutation GPIb-IX complex has been successfully constructed.