Details

Autor(en) / Beteiligte

• Doronina, Lyubov V
• Fedotov, Il'ya V
• Voronin, Aleksandr A
• Ivashkina, Olga I
• Zots, Marina A
• Anokhin, Konstantin V
• Rostova, Ekaterina
• Fedotov, Andrei B
• Zheltikov, Aleksei M

Titel

Tailoring the soliton output of a photonic crystal fiber for enhanced two-photon excited luminescence response from fluorescent protein biomarkers and neuron activity reporters

Ist Teil von

• Optics letters, 2009-11, Vol.34 (21), p.3373

Ort / Verlag

United States

Erscheinungsjahr

2009

Quelle

MEDLINE

Beschreibungen/Notizen

• Dual-cladding photonic crystal fibers (PCFs) with two zero-dispersion points are used to enhance the two-photon excited luminescence (TPL) response from fluorescent protein biomarkers and neuron activity reporters in dye-cell experiments and in in vivo work on transgenic mice and tadpoles. The soliton transmission of ultrashort pulses through a PCF suppresses dispersion-induced temporal pulse spreading, maintaining a high level of field intensity needed for efficient TPL excitation. The soliton self-frequency shift, stabilized against laser power fluctuations by a specific PCF dispersion design, is employed to accurately match the wavelength of the soliton PCF output with the two-photon absorption spectrum of dye or fluorescent protein biomarker molecules, enhancing their TPL response and allowing the laser damage of biotissues to be avoided.