Bingdu xuebao, 2009-03, Vol.25 (2), p.83
2009
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- Autor(en) / Beteiligte
- Titel
- Sequencing and analysis of the full coding sequence of Batai virus isolated in China
- Ist Teil von
- Bingdu xuebao, 2009-03, Vol.25 (2), p.83
- Ort / Verlag
- China
- Erscheinungsjahr
- 2009
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- By RT-PCR and TAIL-PCR, the full coding region of Batai virus isolated in China (YN92-4 strain)was sequenced for the first time. According to the results, the genome of the virus contained three segments S, M and L of 947, 4,371 and 6,860 nucleotides, respectively. The S segment coded a nucleoprotein of 234 amino acids and a nonstructural protein of 102 amino acids, the M and L segments coded a precursor protein of 1 ,435 amino acids and RNA polymerase of 2,239 amino acids, respectively. Compared with the full coding sequence of Batai viruses isolated out of China, the S and M segments of YN92-4 and ON-7/B/01 showed the highest homology in nucleotide and amino acid sequenes with similarity of 97.7% (100%) and 95.7% (98%), respectively. Since there was no full coding sequence information on the L segment in GenBank for the reference, the L segment of YN92-4 was compared with that of Bunyamwera virus and the homology of nucleotide and amino acid was 73.5%and 81.6%, respectively. Phylogenetic analysis showed YN9
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 1000-8721Titel-ID: cdi_pubmed_primary_19678560
- Format
- –
- Schlagworte
- Amino Acid Sequence, Animals, Base Sequence, Bunyamwera virus - genetics, Cattle, China, Clinical Laboratory Techniques, Cloning, Molecular, Genome, Viral - genetics, Hemorrhagic Fever with Renal Syndrome, Molecular Sequence Data, Open Reading Frames, Polymerase Chain Reaction, Reassortant Viruses - genetics, Reverse Transcriptase Polymerase Chain Reaction, Sequence Alignment, Sequence Analysis, DNA