Details

Autor(en) / Beteiligte

• Azuma, Yoshinao
• Hosoyama, Akira
• Matsutani, Minenosuke
• Furuya, Naoko
• Horikawa, Hiroshi
• Harada, Takeshi
• Hirakawa, Hideki
• Kuhara, Satoru
• Matsushita, Kazunobu
• Fujita, Nobuyuki
• Shirai, Mutsunori

Titel

Whole-genome analyses reveal genetic instability of Acetobacter pasteurianus

Ist Teil von

• Nucleic acids research, 2009-09, Vol.37 (17), p.5768-5783

Ort / Verlag

England

Erscheinungsjahr

2009

Quelle

MEDLINE

Beschreibungen/Notizen

• Acetobacter species have been used for brewing traditional vinegar and are known to have genetic instability. To clarify the mutability, Acetobacter pasteurianus NBRC 3283, which forms a multi-phenotype cell complex, was subjected to genome DNA sequencing. The genome analysis revealed that there are more than 280 transposons and five genes with hyper-mutable tandem repeats as common features in the genome consisting of a 2.9-Mb chromosome and six plasmids. There were three single nucleotide mutations and five transposon insertions in 32 isolates from the cell complex. The A. pasteurianus hyper-mutability was applied for breeding a temperature-resistant strain grown at an unviable high-temperature (42 degrees C). The genomic DNA sequence of a heritable mutant showing temperature resistance was analyzed by mutation mapping, illustrating that a 92-kb deletion and three single nucleotide mutations occurred in the genome during the adaptation. Alpha-proteobacteria including A. pasteurianus consists of many intracellular symbionts and parasites, and their genomes show increased evolution rates and intensive genome reduction. However, A. pasteurianus is assumed to be a free-living bacterium, it may have the potentiality to evolve to fit in natural niches of seasonal fruits and flowers with other organisms, such as yeasts and lactic acid bacteria.