Details

Autor(en) / Beteiligte

• Wei, Hong-xia
• Yang, Yi
• Qiu, Hai-bo
• Guo, Tao
• Zhao, Ming-ming
• Chen, Qiu-hua

Titel

Effects of the treatment with different fluids on alveolar epithelium barrier in rats with acute lung injury

Ist Teil von

• Zhongguo wei zhong bing ji jiu yi xue, 2009-07, Vol.21 (7), p.412

Ort / Verlag

China

Erscheinungsjahr

2009

Quelle

MEDLINE

Beschreibungen/Notizen

• To observe the effects of different fluids on alveolar epithelium barrier in rats with acute lung injury (ALI). Thirty-six Sprague-Dawley (SD) rats were randomly assigned into six groups with 6 rats in each group. ALI was induced by intravenous injection of lipopolysaccharide(LPS). Rats in all treatment groups were given different fluids and sacrificed after 4 hours. Evans blue dye (EBD) was injected via the femoral vein 30 minutes before death. Tracheobronchial tree was washed with normal saline (NS) after death, and broncho-alveolar lavage fluid (BALF) was collected. Leakage of EBD from blood into BALF (alveolar epithelial permeability) and wet/dry (W/D) ratio were measured. The mRNA expression of surfactant protein-C (SP-C) was assessed by reverse transcription-polymerase chain reaction (RT-PCR). Alveolar epithelium apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end labeling (TUNEL). Lung injury was evaluated by Smith lung injury score. (1) Lung injury scores in L