Details

Autor(en) / Beteiligte

• Xu, Min-Hua
• Wu, Jia-Ying
• Ou, Li-Li
• Zhu, Dan-Yan
• Yu, Yong-Ping
• Lou, Yi-Jia

Titel

Transcription of cytoskeleton protein genes in differentiation of neurons from mouse embryonic stem cells induced by small molecules

Ist Teil von

• Zhejiang da xue xue bao. Journal of Zhejiang University. Medical sciences. Yi xue ban, 2009-05, Vol.38 (3), p.242

Ort / Verlag

China

Erscheinungsjahr

2009

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• To investigate the transcription of cytoskeleton protein genes in differentiation of neurons from mouse embryonic stem (ES) cells induced by all-trans retinoic acid (RA), and to explore the possibility of setting up a method to screen small molecules with promoting or inhibiting effect. The hanging drop method was employed for embryonic body formation to mimic embryo development in vivo. Reverse transcriptase PCR (RT-PCR) was performed to investigate mRNA expression of the neuron-specific cytoskeleton proteins including Mtap2, Nefm and beta-tubulin III which were regarded as the inducing effect indexes of RA. Morphological evaluation and immunocytochemistry staining were conducted to identify the neural derivatives. Moreover, the inducing effects of six synthetic molecules were further evaluated. RA up-regulated the mRNA expression of Mtap2 and Nefm, especially Mtap2 increased by 1.27 times, which was consistent with the morphological alteration. However, there was no significant changes of beta-tubulin III e