Details

Autor(en) / Beteiligte

• Shen, Yue-di
• Shao, Xue-ting
• Ni, You-di
• Xu, Hang
• Tong, Xiang-min

Titel

Cordyceps sinensis polysaccharide enhances apoptosis of HL-60 cells induced by triptolide

Ist Teil von

• Zhejiang da xue xue bao. Journal of Zhejiang University. Medical sciences. Yi xue ban, 2009-03, Vol.38 (2), p.158

Ort / Verlag

China

Erscheinungsjahr

2009

Quelle

MEDLINE

Beschreibungen/Notizen

• To investigate the effects of polysaccharide fraction of Cordyceps sinensis (PSCS) on triptolide (TPL)-induced apoptosis in the HL-60 cells and the involved molecular mechanism. The cultured leukemia HL-60 cells were divided into three groups: control group, TPL group (cells were treated with 5 ng/ml TPL only), and PSCS+TPL cells group (cells treated with 5 ng/ml TPL and 100 microg/ml or 200 microg/ml PSCS for 18 h). Cell viability was tested by MTT assay and apoptotic cells were quantitatively measured by flow cytometry with Annexin V/PI double stain.The expressions of Caspase-3, 6, 7, 9 and NF-kappa B proteins were tested by Western blot. MTT assay showed that different concentrations of PSCS inhibited the cell viability. Flow cytometry indicated that TPL markedly increased the apoptosis rate of the HL-60 cells, and PSCS enhanced the apoptosis in a dose-dependent manner. Western blot showed that TPL did not inhibit the expression of the Caspase-3, 6, 7, 9 and NF-kappa B proteins, and when cells were treated