Details

Autor(en) / Beteiligte

• Kim, Dongbum
• Kim, Young-Jin
• Seo, Jae-Nam
• Kim, Jinho
• Lee, Younghee
• Park, Cheung-Seog
• Kim, Dae-Won
• Kim, Doo-Sik
• Kwon, Hyung-Joo

Titel

2,4-Dinitrofluorobenzene Modifies Cellular Proteins and Induces Macrophage Inflammatory Protein-2 Gene Expression via Reactive Oxygen Species Production in RAW 264.7 Cells

Ist Teil von

• Immunological investigations, 2009, Vol.38 (2), p.132-152

Ort / Verlag

England: Informa UK Ltd

Erscheinungsjahr

2009

Quelle

MEDLINE

Beschreibungen/Notizen

• The skin sensitizer 2,4-dinitrofluorobenzene (DNFB) provokes delayed hypersensitivity responses as a result of topical application to the skin. Here, we demonstrate that DNFB modifies proteins in RAW 264.7 cells and skin tissues in NC Nga mice; we also show the functional involvement of DNFB-induced modification of cellular proteins in the DNFB-induced macrophage inflammatory protein (MIP)-2 gene expression in RAW 264.7 cells. In addition, we demonstrate that DNFB strongly induces reactive oxygen species (ROS) production. Our RT-PCR analysis and reporter gene assays reveal that the DNFB-induced intracellular ROS production is necessary for MIP-2 gene expression by DNFB. We observed that the vitamin C and chemical oxidant scavenger N-acetyl-cysteine have an inhibitory effect on the generation of ROS, the activation of MAP kinase pathways, and the MIP-2 gene expression in DNFB-treated RAW 264.7 cells. These results provide insight into the mechanisms involved in DNFB-induced contact hypersensitivity.