Details

Autor(en) / Beteiligte

• Cazlaris, H
• Le Marer, N
• Laudet, V
• Lagrou, C
• Zhu, Q
• Delannoy, P
• Montreuil, J

Titel

Modifications of sialylation in BHK 21/C13 cells after in vitro transfection by c-Ha-ras human oncogene

Ist Teil von

• Comptes rendus de l'Académie des sciences, Série III, Sciences de la vie, 1991, Vol.312 (7), p.293

Ort / Verlag

France

Erscheinungsjahr

1991

Quelle

MEDLINE

Beschreibungen/Notizen

• A Hamster kidney fibroblast cell line (BHK 21/C13) has been transfected by c-Ha-ras human oncogene. The expression of the oncogene significantly modified the global sialytransferase activity of the cell extract. This activity is enhanced on an average 2.5 fold whatever the acceptor. In addition, the proportion of cell-surface associated N-glycolylneuraminic acid is enhanced in transfectants, the ratio N-acetylneuraminic acid/N-glycolylneuraminic acid decreases from 7.10 to 2.80. These results suggest that a tight relationship exists, in c-Ha-ras transfected BHK cells, between the expression of the oncogene and the neuraminic acid metabolism as well as a gene regulation of glycoconjugate sialylation.