Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Diabetic cardiomyopathy-associated dysfunction in spatially distinct mitochondrial subpopulations
Ist Teil von
American journal of physiology. Heart and circulatory physiology, 2009-02, Vol.296 (2), p.H359-H369
Ort / Verlag
United States: American Physiological Society
Erscheinungsjahr
2009
Quelle
Free E-Journal (出版社公開部分のみ)
Beschreibungen/Notizen
1 Division of Exercise Physiology, Center for Interdisciplinary Research in Cardiovascular Sciences, West Virginia University School of Medicine, and 2 Department of Basic Pharmaceutical Sciences, West Virginia University School of Pharmacy, Morgantown; and 3 Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia
Submitted 5 May 2008
; accepted in final form 2 December 2008
Diabetic cardiomyopathy is the leading cause of heart failure among diabetic patients, and mitochondrial dysfunction has been implicated as an underlying cause in the pathogenesis. Cardiac mitochondria consist of two spatially, functionally, and morphologically distinct subpopulations, termed subsarcolemmal mitochondria (SSM) and interfibrillar mitochondria (IFM). SSM are situated beneath the plasma membrane, whereas IFM are embedded between myofibrils. The goal of this study was to determine whether spatially distinct cardiac mitochondrial subpopulations respond differently to a diabetic phenotype. Swiss-Webster mice were subjected to intraperitoneal injections of streptozotocin or citrate saline vehicle. Five weeks after injections, diabetic hearts displayed decreased rates of contraction, relaxation, and left ventricular developed pressures ( P < 0.05 for all three). Both mitochondrial size (forward scatter, P < 0.01) and complexity (side scatter, P < 0.01) were decreased in diabetic IFM but not diabetic SSM. Electron transport chain complex II respiration was decreased in diabetic SSM ( P < 0.05) and diabetic IFM ( P < 0.01), with the decrease being greater in IFM. Furthermore, IFM complex I respiration and complex III activity were decreased with diabetes ( P < 0.01) but were unchanged in SSM. Superoxide production was increased only in diabetic IFM ( P < 0.01). Oxidative damage to proteins and lipids, indexed through nitrotyrosine residues and lipid peroxidation, were higher in diabetic IFM ( P < 0.05 and P < 0.01, respectively). The mitochondria-specific phospholipid cardiolipin was decreased in diabetic IFM ( P < 0.01) but not SSM. These results indicate that diabetes mellitus imposes a greater stress on the IFM subpopulation, which is associated, in part, with increased superoxide generation and oxidative damage, resulting in morphological and functional abnormalities that may contribute to the pathogenesis of diabetic cardiomyopathy.
diabetes; free radical; mitochondria
Address for reprint requests and other correspondence: J. M. Hollander, Div. of Exercise Physiology, Center for Interdisciplinary Research in Cardiovascular Sciences, West Virginia Univ. School of Medicine, 1 Medical Center Dr., Morgantown, WV 26506 (e-mail: jhollander{at}hsc.wvu.edu )