Details

Autor(en) / Beteiligte

• Lindshield, Brian L
• King, Jennifer L
• Wyss, Adrian
• Goralczyk, Regina
• Lu, Chi-Hua
• Ford, Nikki A
• Erdman, John W. Jr

Titel

Lycopene Biodistribution Is Altered in 15,15'-Carotenoid Monooxygenase Knockout Mice

Ist Teil von

• The Journal of nutrition, 2008-12, Vol.138 (12), p.2367-2371

Ort / Verlag

Bethesda, MD: American Society for Nutrition

Erscheinungsjahr

2008

Link zum Volltext

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• 15,15'-carotenoid monooxygenase (CMO I) is generally recognized as the central carotenoid cleavage enzyme responsible for converting provitamin A carotenoids to vitamin A, while having little affinity for nonprovitamin A carotenoids, such as lycopene. To investigate the role of CMO I in carotenoid metabolism, ~90-d-old C57BL/6 x 129/SvJ [CMO I wild-type (WT)] and B6;129S6-Bcmo1tm1Dnp [CMO I knockout (KO)] mice were fed a high-fat, moderate vitamin A, cholesterol-containing diet supplemented with 150 mg/kg diet of β-carotene, lycopene, or placebo beadlets for 60 d (n = 12-14). CMO I KO mice fed lycopene (Lyc-KO) exhibited significant decreases in hepatic, spleen, and thymus lycopene concentrations and significant increases in prostate, seminal vesicles, testes, and brain lycopene concentrations compared with WT mice fed lycopene (Lyc-WT). Furthermore, in the serum and all tissues analyzed, excluding the testes, there was a significant increase in the percent lycopene cis isomers in Lyc-KO mice compared with Lyc-WT mice. CMO I KO mice fed β-carotene (βC-KO) had significantly lower hepatic vitamin A concentrations (17% of WT mice fed β-carotene [βC-WT]). Concordantly, βC-KO mice had higher serum and tissue β-carotene concentrations than βC-WT mice. In addition, phenotypically CMO I KO mice had significantly higher final body weights and CMO I KO female mice had significantly lower uterus weights than CMO I WT mice. In conclusion, CMO I KO mice fed low levels of vitamin A have altered lycopene biodistribution and isomer patterns and do not cleave β-carotene to vitamin A at appreciable levels.