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Basolateral tetraethylammonium transport in intact tubules: specificity and trans-stimulation
Ist Teil von
American journal of physiology. Renal physiology, 1991-09, Vol.261 (3), p.386-F392
Ort / Verlag
United States
Erscheinungsjahr
1991
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
W. H. Dantzler, S. H. Wright, V. Chatsudthipong and O. H. Brokl
Department of Physiology, College of Medicine, University of Arizona, Tucson 85724.
To examine the specificity of proximal renal basolateral organic cation
transport, the effects of unlabeled organic cation substrates in the
bathing medium on the rate of uptake [14C]tetraethylammonium ([14C]TEA) by
intact nonperfused proximal tubules and isolated basolateral membrane
vesicles (BLMV) from rabbit kidneys were explored. The pattern of
inhibition of transport by a battery of unlabeled organic cations was
similar in intact tubules and BLMV. To determine if trans-stimulation could
be demonstrated across the basolateral membrane of intact tubules, the
effects of preloading tubules with unlabeled substrates on the rate of
uptake of [14C]TEA and the effects of unlabeled substrates in the bathing
medium on the rate of efflux of [14C]TEA from tubules preloaded with this
labeled substrate were examined. Trans-stimulation was clearly demonstrated
for the first time in intact tubules. However, of the compounds that
significantly inhibited [14C]TEA uptake (TEA, amiloride,
tetrapropylammonium, mepiperphenidol, isopropyl pyridinium, and choline),
only TEA itself and choline produced a trans-stimulation of [14C]TEA
uptake. Moreover, choline appeared to be at least as effective as TEA
itself as a counter ion for TEA transport. Such trans-stimulation could
play a physiological role in the net reabsorption of choline and the net
secretion of most other organic cations.