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Effective cleavage of structured RNAs by tandems of 10-23 DNAzymes with 3'-modified oligo(2'-O-methylribonucleotide)-effectors
Ist Teil von
Nucleic acids symposium series (2004), 2008 (52), p.525
Ort / Verlag
England
Erscheinungsjahr
2008
Quelle
MEDLINE
Beschreibungen/Notizen
Catalytic activity of DNAzymes targeted to IGF-I and MDR1 mRNA can be regulated by the combination of 10-23 DNAzyme with 3'-modified oligo(2'-O-methylribonucleotides) that are favorable as effectors due to their high affinity to RNA and nuclease resistance. We have demonstrated that the DNAzyme constructions designed were able to bind and cleave long structured RNA transcripts effectively under simulated physiological conditions.