Bioscience reports, 2008-12, Vol.28 (6), p.299
2008
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Molecular cloning and characterization of a novel human variant of RIC-3, a putative chaperone of nicotinic acetylcholine receptors
- Ist Teil von
- Bioscience reports, 2008-12, Vol.28 (6), p.299
- Ort / Verlag
- England
- Erscheinungsjahr
- 2008
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Recent reports demonstrate that the RIC-3 (resistant to inhibitors of cholinesterase-3) protein is important for the maturation of nAChRs (nicotinic acetylcholine receptors). In the present study RIC-3e, a novel variant of RIC-3, is described. This variant contains a deletion of exons 4 and 5 of RIC-3, resulting in a protein product lacking a conserved coiled-coil domain. Like RIC-3, the new variant is predominantly, but not exclusively, expressed in the brain. The analysis of expression of variant RIC-3 mRNA and of alpha7-nAChR mRNA in a set of human tissues shows a similar profile. The RIC-3e protein is functionally active and enables surface expression of mature alpha7-nAChRs in cell lines not otherwise permissive for the expression of this receptor.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0144-8463eISSN: 1573-4935DOI: 10.1042/BSR20080055Titel-ID: cdi_pubmed_primary_18691158
- Format
- –
- Schlagworte
- alpha7 Nicotinic Acetylcholine Receptor, Amino Acid Sequence, Animals, CHO Cells, Cloning, Molecular, Cricetinae, Cricetulus, Exons - genetics, Gene Expression Regulation - physiology, Humans, Intracellular Signaling Peptides and Proteins - genetics, Intracellular Signaling Peptides and Proteins - metabolism, Molecular Chaperones - biosynthesis, Molecular Chaperones - genetics, Molecular Sequence Data, Organ Specificity - physiology, Protein Isoforms - biosynthesis, Protein Isoforms - genetics, Protein Structure, Tertiary - genetics, Receptors, Nicotinic - biosynthesis, Receptors, Nicotinic - genetics, RNA, Messenger