Zhonghua nan ke xue, 2008-05, Vol.14 (5), p.391
2008
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Sperm mtDNA content and mtDNA4977bp deletion in normal and leukocytospermia men
- Ist Teil von
- Zhonghua nan ke xue, 2008-05, Vol.14 (5), p.391
- Ort / Verlag
- China
- Erscheinungsjahr
- 2008
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To determine the sperm mtDNA content, mtDNA4977bp deletion and ROS in the seminal plasma of normal and leukocytospermia men, and to investigate the correlation of the changes of sperm mtDNA with the increase of leukocytes and reactive oxgygen species (ROS) in the seminal plasma. Seventy-eight semen samples from leukocytospermia patients and 31 from healthy donors were divided into 3 layers, supernatant fluid, 30% sperm and 80% sperm, by Percoll gradient centrifugation, their sperm mtDNA content and mtDNA4977bp deletion quantitatively analyzed by real-time PCR, and the level of ROS determined by flow cytometry. The ROS in the seminal plasma and the sperm mtDNA contents of the three layers were all significantly higher in the leukocytospermia group than in the healthy control (P < 0.01). In the supernatant fluid and 80% layers, mtDNA4977bp deletion showed no obvious difference between the control and the leukocytospermia group, but was significantly higher in the 30% layer of the latter (P < 0.01). The ROS leve
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 1009-3591Titel-ID: cdi_pubmed_primary_18572854
- Format
- –
- Schlagworte
- Adult, DNA, Mitochondrial - genetics, DNA, Mitochondrial - metabolism, Flow Cytometry, Humans, Infertility, Male - genetics, Infertility, Male - metabolism, Infertility, Male - physiopathology, Leukocytes - chemistry, Leukocytes - metabolism, Leukocytosis - genetics, Leukocytosis - metabolism, Leukocytosis - physiopathology, Male, Polymerase Chain Reaction, Reactive Oxygen Species - metabolism, Sequence Deletion, Sperm Count, Spermatozoa - cytology, Spermatozoa - metabolism