Zhonghua shao shang za zhi, 2008-02, Vol.24 (1), p.9
2008
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- Autor(en) / Beteiligte
- Titel
- Effects of advanced glycosylation end products on the biological behavior of neutrophils
- Ist Teil von
- Zhonghua shao shang za zhi, 2008-02, Vol.24 (1), p.9
- Ort / Verlag
- China
- Erscheinungsjahr
- 2008
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To investigate the effects of advanced glycation end products (AGE) on the biological behavior of neutrophils in vitro, to look for the relationship between accumulation of AGE and abnormal inflammation in wound healing in diabetic mellitus patients. Neutrophils were isolated from SD rats and incubated in vitro. The cells were divided into four groups according to different concentrations of AGE in cell suspension: control group (C, with treatment of RPMI - 1640), A group (with treatment of 0.315 mg/mL AGE + RPMI - 1640), B group (with treatment of 0.625 mg/mL AGE + RPMI - 1640), D group (with treatment of 1.250 mg/mL AGE + RPMI - 1640). Activity of neutrophils were determined by MTT colorimetric assay. Selectin-L mRNA expressions were analyzed by reversible transcription polymerase chain reaction (RT -PCR) technique. The levels of reactive oxygen species (ROS) in neutrophils were measured with DCFH-DA method. The protein concentration of neutrophil elastase (NE) was assayed by ELISA. The activity of neutroph
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 1009-2587Titel-ID: cdi_pubmed_primary_18512551
- Format
- –
- Schlagworte
- Animals, Cells, Cultured, Glycation End Products, Advanced - metabolism, Glycation End Products, Advanced - pharmacology, L-Selectin - metabolism, Leukocyte Elastase - metabolism, Male, Neutrophil Activation, Neutrophils - metabolism, Rats, Rats, Sprague-Dawley, Reactive Oxygen Species - metabolism