Yao hsüeh hsüeh pao, 2008-02, Vol.43 (2), p.138
2008
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- Autor(en) / Beteiligte
- Titel
- Induction of G2 /M phase arrest and apoptosis of MCF-7 cells by novel benzofuran lignan via suppressing cell cycle proteins
- Ist Teil von
- Yao hsüeh hsüeh pao, 2008-02, Vol.43 (2), p.138
- Ort / Verlag
- China
- Erscheinungsjahr
- 2008
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- In the present study, a newly synthesized benzofuran lignan 4-formyl-2-(4-hydroxy-3methoxyphenyl)-5-(2-methoxycarbonyethyl)-7-methoxy-benzo [b] furan (ERJT-12) was tested for its antiproliferative activity on human tumor cells. The related mechanisms were also investigated. In vitro growth inhibitory effects of ERJT-12 on various cancer cell lines were determined by MTT assay. Cell cycle distribution and apoptosis were detected by flow cytometry. The integrity of DNA was assessed by agarose gel electrophoresis. Activation of Caspase-3/7 and Caspase-6 was measured by colorimetric assay. The expressions of cell cycle proteins cell divide cycle 25c (Cdc25c), cyclin dependent kinase 1 (CDK1), CyclinB1 and apoptosis-related proteins Bax and Bcl-2 were detected by Western blotting. MTT assay showed that ERJT-12 inhibited the proliferation of several cancer cell lines including multidrug resistant cells. MCF-7 cells were markedly arrested at gap2/mitosis (G2/M) phase after treatment with ERJT-12 and progressed into
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 0513-4870Titel-ID: cdi_pubmed_primary_18507339
- Format
- –
- Schlagworte
- Antineoplastic Agents - pharmacology, Apoptosis - drug effects, bcl-2-Associated X Protein - metabolism, Benzofurans - pharmacology, Caspase 3 - metabolism, Caspase 6 - metabolism, Caspase 7 - metabolism, CDC2 Protein Kinase - metabolism, cdc25 Phosphatases - metabolism, Cell Cycle Proteins - metabolism, Cell Division - drug effects, Cell Line, Tumor, Cyclin B - metabolism, Cyclin B1, G2 Phase - drug effects, Humans, Proto-Oncogene Proteins c-bcl-2 - metabolism