Details

Autor(en) / Beteiligte

• Sun, Guo-zhu
• Hu, Qing-shan
• Zhang, Qing-jun
• Zhao, Zong-mao
• Zhang, Geng-shen

Titel

Changes of protein kinase C activity in experimental presyrinx state in rabbits

Ist Teil von

• Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2008-04, Vol.28 (4), p.582

Ort / Verlag

China

Erscheinungsjahr

2008

Quelle

MEDLINE

Beschreibungen/Notizen

• To investigate the changes of protein kinase C (PKC) activity and its role in the development of presyrinx state in rabbits. Presyrinx state was established in 56 rabbits by intra-cisternal injection of kaolin. At 1, 3, 7, 14, and 21 days after the injection, the water content in the upper cervical spinal cord was measured, its pathological changes observed microscopically and the PKC activity determined with substrate phosphorolysis kinase assay. Spinal cord edema occurred in rabbits one day after kaolin injection, with water content of (68.35-/+0.70)%, which increased to (72.70-/+0.88)% on day 3, reaching the peak level of (72.92-/+0.86)% on day 7, followed by gradual decline after 3 weeks [(70.03-/+0.77)%]. The membrane PKC activity increased from 5.67-/+0.26 pmol.mg(-1).min(-1) on day 1 after the injection to reach the peak level on day 7 (13.27-/+3.15 pmol.mg(-1).min(-1)), which was maintained till day 14 with subsequent decrease to 8.85-/+1.56 pmol.mg(-1).min(-1) on day 21. The cytoplasmic PKC activity