Details

Autor(en) / Beteiligte

• Affolabi, D
• Odoun, M
• Sanoussi, C N
• Tanimomo-Kledjo, B
• Saïzonou, D
• Soumaïla, K
• Kestens, L
• Anagonou, S Y
• Portaels, F

Titel

Bulk staining of smears: no demonstrated risk of bacilli transfer from a positive to a negative smear

Ist Teil von

• The international journal of tuberculosis and lung disease, 2008-06, Vol.12 (6), p.683

Ort / Verlag

France

Erscheinungsjahr

2008

Quelle

MEDLINE

Beschreibungen/Notizen

• Despite a theoretical risk of transfer of bacilli from a positive to a negative smear, bulk staining is routinely performed in many laboratories. To assess this risk in our laboratory, two smears were made from each sputum specimen and stained with auramine: one smear was stained on a rack and the second using the bulk method. Smears were read blind using a fluorescence microscope. A total of 811 sputum specimens were analysed. No acid-fast bacilli transfer was observed even when staining solution jars had not been renewed for 3 days. Bulk staining is rapid and cheap, and could be used in laboratories with a high workload in low-resource settings.