Details

Autor(en) / Beteiligte

• Guo, Yong
• Han, Ying
• Quan, Guo-Bo
• Liu, Min-Xia
• Liu, An

Titel

Morphology, ultrastructure and function of glycosylation-modified chilled blood platelets

Ist Teil von

• Zhongguo shi yan xue ye xue za zhi, 2008-04, Vol.16 (2), p.411

Ort / Verlag

China

Erscheinungsjahr

2008

Quelle

MEDLINE

Beschreibungen/Notizen

• The glycosylation of platelets may prolong their life-span when being transfused after preservation under 4 degrees C, therefore this study was aimed to investigate the effect of glycosylation on morphology, ultrastructure, function and membrane glycoprotein of platelets. The experiments were divided into 3 groups: group preserved in room temperature (RT group), group preserved in 4 degrees C (4T group) and group UDP-Gal glycosylated and preserved in 4 degrees C (U+4T group). The binding rate of RCA I lectin and expression of platelet surface markers CD62P, CD42b were determined by flow cytometry. Morphology and ultrastructure of platelets were observed by light microscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Platelets aggregation was detected by aggregometer. The results showed that the binding rate of RCAI in U+4T group significantly higher than that in RT group (p<0.01), no obvious changes was found in ultrastructure of glycosylated platelets, as compared with fre