Details

Autor(en) / Beteiligte

• Jiang, Dapeng
• Jiang, Zhitao
• Han, Fuyou
• Zhang, Yubo
• Li, Zhaozhu

Titel

HGF suppresses the production of collagen type III and alpha-SMA induced by TGF-beta1 in healing fibroblasts

Ist Teil von

• European journal of applied physiology, 2008-07, Vol.103 (5), p.489

Ort / Verlag

Germany

Erscheinungsjahr

2008

Quelle

MEDLINE

Beschreibungen/Notizen

• The aim of this study was to examine the effectiveness of HGF in blocking TGF-beta1-induced collagen III and alpha-smooth muscle actin (alpha-SMA) production in rat healing fibroblasts, fibroblasts were obtained from healing medial collateral ligament (MCL) injury. Cell culture was supplemented with 5 ng/ml of TGF-beta1 along with increasing doses of HGF (10-40 ng/ml). The productions of collagen III in supernatants culture were assayed by enzyme-linked immunosorbent assay. Expression of alpha-SMA was assessed by Western blot. Treatment with TGF-beta1 significantly stimulated collagen III and alpha-SMA production in healing fibroblasts. Remarkably, the addition of HGF reduced productions of all components induced by TGF-beta1 in a dose-dependent manner. This study shows that HGF antagonizes the action of TGF-beta1 effectively in cultured healing MCL injury fibroblasts. The results provide a cellular and molecular basis for HGF's acting as a therapeutic agent for MCL scar formation and poor healing.