Details

Autor(en) / Beteiligte

• Nedelkov, Dobrin
• Kiernan, Urban A
• Niederkofler, Eric E
• Tubbs, Kemmons A
• Nelson, Randall W

Titel

Volumetric mass spectrometry protein arrays

Ist Teil von

• Methods in molecular biology (Clifton, N.J.), 2007, Vol.382, p.333

Ort / Verlag

United States

Erscheinungsjahr

2007

Quelle

MEDLINE

Beschreibungen/Notizen

• Affinity mass spectrometry is a proteomics approach for selectively isolating target proteins from complex biological fluids for mass spectrometric analysis. When executed in high throughput mode through affinity pipets, the resulting volumetric mass spectrometry arrays enable rapid protein assaying from hundreds of samples. Furthermore, in combination with postcapture proteolytic degradation, this top-down proteomics approach can reveal structural features (i.e., modifications) in the protein sequences that are result of posttranslational modifications and/or point mutations. Described here in greater detail are the individual steps of the high throughput combination of affinity protein capture in antibody-derivatized affinity pipets, protein elution, and protein processing through enzyme-derivatized mass spectrometry targets.