Details

Autor(en) / Beteiligte

• Jakimowicz, Dagmara
• Brzostek, Anna
• Rumijowska-Galewicz, Anna
• Żydek, Paulina
• Dołzbłasz, Alicja
• Smulczyk-Krawczyszyn, Aleksandra
• Zimniak, Tomasz
• Wojtasz, Łukasz
• Zawilak-Pawlik, Anna
• Kois, Agnieszka
• Dziadek, Jarosław
• Zakrzewska-Czerwińska, Jolanta

Titel

Characterization of the mycobacterial chromosome segregation protein ParB and identification of its target in Mycobacterium smegmatis

Ist Teil von

• Microbiology (Society for General Microbiology), 2007-12, Vol.153 (Pt 12), p.4050-4060

Ort / Verlag

England

Erscheinungsjahr

2007

Quelle

MEDLINE

Beschreibungen/Notizen

• Bacterial chromosomes (though not Escherichia coli and some other gamma-proteobacterial chromosomes) contain parS sequences and parAB genes encoding partitioning proteins, i.e. ParA (ATPase) and ParB (DNA-binding proteins) that are components of the segregation machinery. Here, mycobacterial parABS elements were characterized for the first time. parAB genes are not essential in Mycobacterium smegmatis; however, elimination or overexpression of ParB protein causes growth inhibition. Deletion of parB also leads to a rather severe chromosome segregation defect: up to 10% of the cells were anucleate. Mycobacterial ParB protein uses three oriC-proximal parS sequences as targets to organize the origin region into a compact nucleoprotein complex. Formation of such a complex involves ParB-ParB interactions and is assisted by ParA protein.