Xi bao yu fen zi mian yi xue za zhi, 2007-09, Vol.23 (9), p.804
2007
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Details
- Autor(en) / Beteiligte
- Titel
- Screening of polypeptides interacting with Rel homology domain of NF-kappaB p50 subunit
- Ist Teil von
- Xi bao yu fen zi mian yi xue za zhi, 2007-09, Vol.23 (9), p.804
- Ort / Verlag
- China
- Erscheinungsjahr
- 2007
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To obtain the polypeptides interacting with NF-kappaB p50 Rel homology domain (RHD) by yeast two-hybrid technique. Using NF-kappaB p50 RHD as bait, the polypeptides interacting with NF-kappaB p50 RHD were screened from a 16-peptide cDNA library by yeast two-hybrid technique. The false positive clones were screened out but the positive clone were identified by beta-GAL assay, yeast mating, and one to one yeast two-hybrid method. 8 positive clones were obtained. The gene sequences of the eight polypeptides were different from each other and their homologous proteins were not found in GenBank. 8 novel polypeptides interacting with NF-kappaB p50 RHD were obtained, but their functions need to be validated in further experiments.
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 1007-8738Titel-ID: cdi_pubmed_primary_17825223
- Format
- –
- Schlagworte
- Amino Acid Sequence, Databases, Genetic, Genes, Reporter, Lac Operon, Molecular Sequence Data, NF-kappa B p50 Subunit - chemistry, NF-kappa B p50 Subunit - metabolism, Peptides - analysis, Peptides - chemistry, Peptides - metabolism, Plasmids - analysis, Plasmids - genetics, Plasmids - metabolism, Protein Structure, Tertiary, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Transcription Factor RelA - metabolism, Transcription Factor RelB - metabolism, Two-Hybrid System Techniques