Details

Autor(en) / Beteiligte

• Zhang, Rui-Li
• Niu, Xu-Ping
• Li, Xin-Hua
• Zhang, Kai-Ming
• Yin, Guo-Hua

Titel

CFU-HPP colony formation of bone marrow hematopoietic proginitor cells in psoriatic patients and methylation of p16 gene promotor in CFU-HPP colony cells

Ist Teil von

• Zhongguo shi yan xue ye xue za zhi, 2007-08, Vol.15 (4), p.780

Ort / Verlag

China

Erscheinungsjahr

2007

Quelle

MEDLINE

Beschreibungen/Notizen

• This study was purposed to investigate the colony formation of high-proliferative potential colony-forming units (CFU-HPP) from bone marrow-derived hematopoietic cells of psoriatic patients and p16 gene promotor methylation in CFU-HPP cells, and to explore the relationship between the colony formation and the methylation status of p16 gene promoter. Bone marrow-derived mononuclear cells from psoriatic patients and normal controls were separated by density gradient centrifugation, and were cultured in methycellulose semi-solid culture medium with SCF, GM-CSF, IL-3 and IL-6 for 14 days to measure the colonies of CFU-HPP. The CFU-HPP colony cells were collected and methylation status of p16 gene promoter of CFU-HPP cell DNA modified with sodium bisulfite was detected by the methylation-specific polymerase chain reaction (MSP). The results showed that in methycellulose semi-solid culture system, the number and the size of CFU-HPP colonies of bone marrow of psoriatic patients were all significantly less than that